A RAPID GENOTYPING METHOD FOR AN OBLIGATE FUNGAL PATHOGEN, PUCCINIA STRIIFORMIS F.SP. TRITICI, BASED ON DNA EXTRACTION FROM INFECTED LEAF AND MULTIPLEX PCR GENOTYPING

Abstract Background Puccinia striiformis f.sp.tritici (PST), an obligate fungal pathogen causing wheat yellow/stripe rust, a serious disease, has been used to understand the evolution of crop pathogen using molecular markers.However, numerous questions regarding its evolutionary history and recent migration routes still remains to be addressed, whi

In the development of practically all potash salt deposits, the study of gas-dynamic phenomena (GDP) is one of the most difficult tasks to ensure mining safety.Sudden salt and gas outbursts, dynamic breakdown, which are accompanied by intense gas release and possible broken rock carry-over into the mine workings, are associated with GDP.Geological

Abstract Background Ichang papeda (Citrus ichangensis), a wild perennial plant of the Rutaceae family, is a cold-hardy plant.WRKY transcription factors are crucial regulators Nursing Covers of plant growth and development as well as abiotic stress responses.However, the WRKY genes in C.ichangensis (CiWRKY) and their expression patterns under cold s

Abstract Background The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has become a powerful tool for functional genomics in plants.The Eksemcreme RNA-guided nuclease can be used to not only generate precise genomic mutations, but also to manipulate gene expression when present as a deactivated protein (dCas9).Result

Purpose: This manuscript explores the link between Spare Parts Management and Total Costs of Ownership or Life Cycle Costs (LCC).Design/methodology/approach: First, this work enumerates the different managerial decisions instances in spare parts management that are present during the life cycle of a physical asset.Second, we analyse how those decis